HDAC4 (aa 612-1084) Protein (GST Tag)

16.04.2012

Produkt-Information von HDAC4 Protein

Synonym	AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA
Protein Construction	A DNA sequence encoding the amino acid sequence (Met 612-Leu 1084) of human HDAC4 (P56524), that is the the histone deacetylase region, was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Source	Human
Expression Host	Baculovirus-Insect cells

HDAC4 Protein QC Testing

Purity	> 92 % as determined by SDS-PAGE	SDS-PAGE: HDAC4 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human HDAC4 (aa 612-1084)/GST chimera consists of 711 amino acids and has a calculated molecular mass of 78.5KDa. It migrates as an approximately 72 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 20mM Tris,500mM NaCl, pH 7.4, 20%glycerol,3mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von HDAC4 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von HDAC4 Protein

HDAC4 (histone deacetylase 4), belongs to class II of the histone deacetylase/acuc/apha family. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone Deacetylases (HDACs) are closely related to sirtuins. They usually act as components of large multiprotein complexes. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones. Histone deacetylation gives a tag for epigenetic repression. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

References

Geng H. et al., 2011, J Biol Chem. 286 (44): 38095-102.
Yuan JH. et al., 2011, Hepatology. 54 (6): 2025-35.
Cernotta N. et al., 2011, Mol Biol Cell. 22 (2): 278-89.

Sino Biological Inc. liefert HDAC4 (aa 612-1084) Protein (GST Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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