GRK5 Antikörper (Antigen Affinität reinigt)

14.02.2012

Produkt-Information von GRK5 Antikörper

Immunogen	Recombinant Human GRK5 protein (Catalog#10839-H08B)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human GRK5 (rh GRK5;Catalog#10839-H08B; NP_005299.1; Met 1-Ser 590). GRK5 specific IgG was purified by Human GRK5 affinity chromatography.

GRK5 Antikörper Usage Guide

Specificity	Human GRK5
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GRK5 in WB. Using a DAB detection system, the detection limit for Human GRK5 is approximately 4 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GRK5. The detection limit for Human GRK5 is approximately 0.039 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von GRK5 Antikörper

Verwandte Bereiche:

Proteine:

Antikörper:

Hintergrund von GRK5 Antikörper

G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors. GRK5 contains oneAGC-kinase C-terminal domain, oneprotein kinase domain and oneRGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease.

References

Kunapuli P. et al., 1994, J Biol Chem. 269 (14): 10209-12.
Millman EE. et al., 2004, Br J Pharmacol. 141 (2): 277-84.
Thiyagarajan MM. et al., 2004, J Biol Chem. 279 (17): 17989-95.
Suo Z. et al., 2007, Neurobiol Aging. 28 (12): 1873-88.
Li L. et al., 2008, J Neuroinflammation. 5: 24.

Sino Biological Inc. liefert GRK5 Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über GRK5 Antikörper (Antigen Affinität reinigt) lesen Sie bitte: http://www.sinobiological.com/goods.php?id=7604