GPA33 Antikörper (Antigen Affinität reinigt)

02.03.2012

Produkt-Information von GPA33 Antikörper

Immunogen	Recombinant Mouse GPA33 protein (Catalog#50559-M08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Mouse GPA33 (rM GPA33;Catalog#50559-M08H; NP_067623.1; Met 1-Ile 235). GPA33 specific IgG was purified by Mouse GPA33 affinity chromatography.

GPA33 Antikörper Usage Guide

Specificity	Mouse GPA33
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse GPA33 in WB. Using a DAB detection system, the detection limit for Mouse GPA33 is approximately 0.5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse GPA33. The detection limit for Mouse GPA33 is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von GPA33 Antikörper

Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.

References

Heath J.K., et al., 1997, Proc. Natl. Acad. Sci. USA. 94: 469-74.
Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236: 682-6.
Frey,D. et al., 2008, Cancer Biother Radiopharm. 23 (1): 65-73.
Rageul,J. et al., 2009,Int J Cancer. 125 (12): 2802-9.

Sino Biological Inc. liefert GPA33 Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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