GOT1 / Aspartate aminotransferase Protein (His Tag)

22.04.2013

Sino Biological Inc. liefert GOT1 / Aspartate aminotransferase Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über GOT1 / Aspartate aminotransferase Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/Aspartate-aminotransferase-GOT1-Protein-Antibody-a-5853.html

Produkt-Information von GOT1 / Aspartate aminotransferase Protein

Synonym	GOT1
Protein Construction	A DNA sequence encoding the mature form of human GOT1 (P17174) (Met1-Gln413) was expressed with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

QC Testing von GOT1 / Aspartate aminotransferase Protein

Purity	> 90% as determined by SDS-PAGE	SDS-PAGE: GOT1 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	His
Molecular Mass	The recombinant human GOT1 consists of 428 amino acids and predicts a molecular mass of 48.1 KDa. It migrates as an approximately 36-45 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, PH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.

Usage Guide von GOT1 / Aspartate aminotransferase Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von GOT1 / Aspartate aminotransferase Protein

Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.

References

Shen H. et al., 2011, J Hum Genet. 56 (11): 801-5.
Doonan S. et al., 1985, Int J Biochem. 16 (12): 1193-9.
Panteghini M. 1990, Clin Biochem. 23 (4): 311-9.
Bousquet-Lemercier B. et al., 1990, Biochemistry. 29 (22): 5293-9.

Aspartate aminotransferase (GOT1) related areas, pathways, and other information