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| Immunogen | Recombinant Human GOLM1 / GP73 protein (Catalog#13066-H07E) |
| Antibody Type | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
| Clone ID | 11F1A7 |
| Formulation | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Catalog#13066-H07E; Q8NBJ4-1; Met 1-Leu 401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Specificity | Human GOLM1 / GP73 |
| No cross-reactivity in ELISA with | |
| Western blot | This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human GP73 in WB. Using a DAB detection system, the detection limit for Human GP73 is approximately 20 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended. |
| Direct ELISA | This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human GP73. The detection limit for Human GP73 is approximately 0.039 ng/well. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2 and GOLM1, is a protein which belongs to theGOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in colon, prostate, trachea and stomach. It is expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncitial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
Sino Biological Inc. liefert GOLM1 / GP73 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.
Weitere Informationen über GOLM1 / GP73 Antikörper lesen Sie bitte: http://www.sinobiological.com/GOLM1-GP73-Antibody-g-8702.html
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