Galactolipase / PNLIPRP2 Protein (His Tag)

21.12.2012

Sino Biological Inc. liefert Galactolipase / PNLIPRP2 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über Galactolipase / PNLIPRP2 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/Galactolipase-PNLIPRP2-Protein-g-10173.html

Produkt-Information von Galactolipase / PNLIPRP2 Protein

Synonym	PNLIPRP2,PLRP2
Protein Construction	A DNA sequence encoding the human PNLIPRP2 (P54317)( Met 1- Cys469) was expressed, with a C-terminal polyhistidine tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von Galactolipase / PNLIPRP2 Protein

Purity	> 95 % as determined by SDS-PAGE	SDS-PAGE: PNLIPRP2 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Lys 18
Molecular Mass	The secreted recombinant human PNLIPRP2 consists of 462 amino acids and predicts a molecular mass of 51.5 KDa. The apparent molecular mass of the protein is approximately 57 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 20mM Tris,500mM NaCl, pH 7.4, 10% gly. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von Galactolipase / PNLIPRP2 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von Galactolipase / PNLIPRP2 Protein

Galactolipase, also known as PNLIPRP2, is a lipase with broad substrate specificity. Galactolipase contributes to milk fat hydrolysis. It hydrolyses milk fat with a lower catalytic efficiency. The expressed galactolipase shows a lipolytic activity that is, however, only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase. Galactolipase also hydrolyzes both phospholipids and galactolipids. It acts preferentially on monoglycerides, phospholipids and galactolipids.

References

Andersson EL. et al., 2011, J Lipid Res. 52 (11): 1949-56.
Xiao X. et al., 2011, J Biol Chem. 286 (30): 26353-63.
Alves BN. et al., 2009, Cell Biochem Funct. 27 (5): 296-308.