Mein Blog umfasst die aktuellesten News, Forschungsleistung, neuesten Produkte und Service von Sino Biological Inc. und die aktuelle News von Life Sciences. Sie können sich hier wissenschaftlich informieren.Willkommen zu meinem Blog.Viel Spaß!
| Immunogen | Recombinant Human FGFR4 / CD334 protein (Catalog#10538-H08H) |
| Antibody Type | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
| Clone ID | 01 |
| Ig Type | Mouse IgG1 |
| Formulation | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human FGFR4 / CD334 (rh FGFR4 / CD334; Catalog#10538-H08H; NP_002002.3; Met 1-Asp 369). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Specificity | Human FGFR4 / CD334 |
| No cross-reactivity in ELISA with Human FGFR1 Human FGFR2 Mouse FGFR3 Human cell lysate (293 cell line) | |
| Western blot | This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human FGFR4 in WB. Using a DAB detection system, the detection limit for Human FGFR4 is approximately 10 ng/lane under non-reducing conditions and 50 ng/lane under reducing conditions. |
| Direct ELISA | This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human FGFR4. The detection limit for Human FGFR4 is approximately 0.16 ng/well. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
FGFR4, also designated as CD334, is a member of the fibroblast growth factor receptor subfamily which belongs to the tyrosine protein kinase superfamily. Upon ligand binding, FGFRs undergo dimerization and autophosphorylation, intiating a cascade of downstream signal pathways, ultimately influencing a variety of physiological and pathological cellular processes including mitogenesis and differentiation. FGFR members is highly conserved in amino acid sequence and differ f in their ligand affinities and tissue distribution. FGFR4, a type I transmembrane glycoprotein, contains 3 immunoglobulin-like domains in the extracellular domain and a cytoplasmic tyrosine-kinase domain. FGFR4 expression is detected in gastrointestinal epithelial cells, pancreas, as well as gastric and pancreatic cancer cell lines. FGFR4 is reported to be a high affinity receptor for both acidic and basic fibroblast growth factor, and also binds to FGF19. Like FGF R1- 3,several isoforms of FGFR4 are generated by alternative splicing of the mRNAs. Although its specific function is unknown, growing studies have indicated that FGFR4 plays a role in tumorigenesis and malignace of cetain carcinomas such as breast and ovarian tumors.
Sino Biological Inc. liefert FGFR4 / CD334 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.
Weitere Informationen über FGFR4 / CD334 Antikörper lesen Sie bitte: http://www.sinobiological.com/FGFR4-CD334-Antibody-g-9170.html
/https%3A%2F%2Fassets.over-blog.com%2Ft%2Fcedistic%2Fcamera.png)