Escherichia coli acnB Antikörper

14.03.2012

Produkt-Information von Escherichia coli acnB Antikörper

Immunogen	Recombinant Escherichia coli acnB protein (Catalog#13102-ENAE)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Escherichia coli acnB (Catalog#13102-ENAE; Met 1-Val 865). Total IgG was purified by Protein A affinity chromatography.

Escherichia coli acnB Antikörper Usage Guide

Specificity	Escherichia coli acnB
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect E. coli AcnB in WB.
Direct ELISA	This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect E. coli AcnB. The detection limit for E. coli AcnB is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von Escherichia coli acnB Antikörper

Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.

References

Bradbury AJ. et al., 1996, 142 (Pt 2): 389-400.
Cunningham L. et al., 1997, Microbiology. 143 (Pt 12): 3795-805.
Jordan PA. et al., 1999, Biochem J. 344 Pt 3: 739-46.
Williams CH. et al., 2002, Nat Struct Biol. 9 (6): 447-52.

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