EphB4 Antikörper (Antigen Affinität reinigt)

02.03.2012

Produkt-Information von EphB4 Antikörper

Immunogen	Recombinant Mouse EphB4 protein (Catalog#50582-M08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Mouse EphB4 (rM EphB4;Catalog#50582-M08H; P54761-1; Met 1-Ala 539). EphB4 specific IgG was purified by Mouse EphB4 affinity chromatography.

EphB4 Antikörper Usage Guide

Specificity	Mouse EphB4
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse EPHB4 in WB. Using a DAB detection system, the detection limit for Mouse EPHB4 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse EPHB4. The detection limit for Mouse EPHB4 is approximately 0.00245 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von EphB4 Antikörper

EphB4, also known as Htk, Myk-1, is a member of the Eph subfamily of receptor tyrosine kinases (RTKs). The Ephs are divided into two classes based on their extracellular structure and ligand specificity, and the transmembrane receptor binding preferentially to ephrin-B ligands is referred to as EphB. Sharing common structure organization, all Eph receptors contain an N-terminal Ig-like domain, a cysteine-rich region and two fibronectin type III domains within the ECD, as well as the typical cytoplasmic tyrosine kinase domain. EphB4 and its ligand ephrin-B2 are specifically expressed on venous and arterial endothelial cells, respectively, and play an essential role in vascular development via bidirectional signals. The forward EphB4 signaling inhibits cell adhesion, chemotaxis, angiogenesis, and tumor growth. In contrast, the reverse Ephrin-B2 signaling exerts the opposite effect. It has been reported that aberrant expression of EphB4 is associated with prostate cancer and highly malignant breast cancers, and accordingly, EphB4 has potential applications as a therapeutic candidate.

References

Bennett, B.D. et al., 1994, J. Biol. Chem. 269: 14211-14218.
Noren, N.K. et al., 2004, Proc. Natl. Acad. Sci. 101: 5583-5588.
Murai, K.K. et al., 2003, J. Cell. Sci. 116: 2823-2832.
Noren, N.K. et al., 2006, Nat. Cell. Biol. 8: 815-825.
Xia, G. et al., 2005, Cancer Res. 65: 4623-4632.
Kertesz, N. et al., 2006, Blood. 107: 2330-2338.

Sino Biological Inc. liefert EphB4 Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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