EphA2 Antikörper

01.03.2012

Produkt-Information von EphA2 Antikörper

Immunogen	Recombinant Mouse EphA2 protein (Catalog#50586-M08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Mouse EphA2 (rM EphA2;Catalog#50586-M08H; NP_034269.2; Met 1-Asn 535). Total IgG was purified by Protein A affinity chromatography.

EphA2 Antikörper Usage Guide

Specificity	Mouse EphA2
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse EPHA2 in WB.
Direct ELISA	This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse EPHA2. The detection limit for Mouse EPHA2 is approximately 0.0195 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von EphA2 Antikörper

Ephrin type-A receptor 2, also known as Epithelial cell kinase, Tyrosine-protein kinase receptor ECK and EPHA2, is a single-pass type I membrane protein which belongs to theprotein kinase superfamily, tyr protein kinase family and ephrin receptor subfamily. EPHA2 is a receptor for members of the ephrin-A family. EPHA2 binds to ephrin-A1, ephrin-A3, ephrin-A4 and ephrin-A5. It plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2. The ephrins (Eph) and Eph receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosyl- phosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. In molecular biology, ephrins and Eph receptors are components of cell signaling pathways involved in animal development, and implicated in some cancers. Eph receptors are classified as receptor tyrosine kinases (RTKs), and form the largest sub-family of RTKs.

References

Nowakowski J., et al., 2002, Structure 10: 1659-67.
Murai, KK. et al., 2003,Journal of Cell Science.116 (Pt 14): 2823-32.
The MGC Project Team. 2004, Genome Res. 14: 2121-7.
Rikova K., et al., 2007, Cell 131: 1190-203.
Zhang G., et al., 2008, Cancer Res. 68: 1691-6.
Himanen J.P., et al., 2009, EMBO Rep. 10: 722-8.
Heibeck TH., et al., 2009, J. Proteome Res. 8: 3852-61.

Sino Biological Inc. liefert EphA2 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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