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| Immunogen | Recombinant Rat E-Selectin / CD62e / SELE protein (Catalog#80033-R08H) |
| Antibody Type | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
| Clone ID | 04 |
| Ig Type | Mouse IgG1 |
| Formulation | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat E-Selectin / CD62e / SELE (rR E-Selectin / CD62e / SELE; Catalog#80033-R08H; P98105-1; Met 1-Pro 494). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Specificity | Rat E-Selectin / CD62e / SELE |
| No cross-reactivity in ELISA with Human cell lysate (293 cell line) | |
| Western blot | This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Rat SELE in WB. Using a DAB detection system, the detection limit for Rat SELE is approximately 20 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions. |
| Direct ELISA | This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rat SELE. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed at relatively high density on the surface of cultured endothelial cells that have been activated by proinflammatory cytokines, such as IL-1β, TNF-α, or bacterial endotoxin. E-Selectin/CD62e exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-Selectin/CD62e recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-Selectin/CD62e supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. In addition, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.
Sino Biological Inc. liefert E-Selectin / CD62e / SELE Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.
Weitere Informationen über E-Selectin / CD62e / SELE Antikörper lesen Sie bitte: http://www.sinobiological.com/E-Selectin-CD62e-SELE-Antibody-g-9177.html
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