CNPY4 Protein (His Tag)

20.12.2012

Sino Biological Inc. liefert CNPY4 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über CNPY4 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/CNPY4-Protein-g-10170.html

Produkt-Information von CNPY4 Protein

Synonym	CNPY4
Protein Construction	A DNA sequence encoding the human CNPY4 (Q8N129) (Met1-Leu248) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von CNPY4 Protein

Purity	> 85% as determined by SDS-PAGE	SDS-PAGE: CNPY4 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Gly 22
Molecular Mass	The recombinant human CNPY4 consists of 238 amino acids and predicts a molecular mass of 27.4 KDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CNPY4 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CNPY4 Protein

CNPY4 is a member of the canopy family. CNPY4 interacts with toll-like receptor 4 (TLR4) and plays a role in the regulation of the cell surface expression of TLR4. Toll-like receptors (TLRs) recognize microbial products and induce immune responses. As CNPY4, PRAT4B also regulates cell surface expression of TLR4. PRAT4B has a signal peptide followed by a mature peptide. It is associated with the hypoglycosylated, immature form of TLR4. Lipopolysaccharide is recognized by the receptor complex consisting of TLR4 and MD-2.

References

Stelzl U. et al., 2005, Cell. 122 (6): 957-68.
Hocking JC. et al., 2010, Mech Dev. 127 (1-2): 36-48.
Hart BE. et al., 2012, J Biol Chem. 287 (20): 16550-62.