CD8a Antikörper

27.06.2012

Proudkt-Information von CD8a Antikörper

Immunogen	Recombinant Ferret CD8a protein (Catalog#60001-F08H)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	3B5H2
Ig Type	Mouse IgG1
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret CD8a (rF CD8a; Catalog#60001-F08H; ABS50091.1; Met 1-Glu 186). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Usage Guide von CD8a Antikörper

Specificity	Ferret CD8a
	No cross-reactivity in ELISA with Human CD8A Mouse CD8A Human cell lysate (293 cell line)
Western blot	This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Ferret CD8A in WB. Using a DAB detection system, the detection limit for Ferret CD8A is approximately 2 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Ferret CD8A. The detection limit for Ferret CD8A is approximately 0.078 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von CD8a Antikörper

Related Areas:

Immunology>>Cluster of Differentiation>>CD8a

Proteins:

Molecule	Species	Description //For Detailed Info. and Price------CLICK!	Cat. No
CD8a	Human	CD8a Protein, Recombinant	10980-H08H
CD8a	Mouse	CD8a Protein, Recombinant	50389-M08H
CD8a	Ferret	CD8a Protein, Recombinant	60001-F08H

Antibodies:

Molecule	Application	Description //For Detailed Info. and Price------CLICK!	Cat. No
Human CD8a	WB, ELISA	CD8a Antibody, Mouse Mab	10980-MM04
Mouse CD8a	WB, ELISA	Rabbit Polyclonal Antibody	50389-RP01
Mouse CD8a	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	50389-RP02
Mouse CD8a	WB, ELISA	CD8a Antibody, Rabbit MAb	50389-R012
Mouse CD8a	FCM	CD8a Antibody ( FITC )	50389-R208-F
Mouse CD8a	WB, ELISA	CD8a Antibody	60001-MM02

Hintergrund von CD8a Antikörper

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

References

Devine, L. et al., 2000, J Immunol. 164 (2): 833-8.
Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76.
Saha, K. et al., 2001, Nat Med. 7 (1): 65-72.
Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.

Sino Biological Inc. liefert CD8a Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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