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| Immunogen | Recombinant Cynomolgus CD32a / Fc gamma RIIA protein (Catalog#90015-C08H) |
| Antibody Type | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
| Clone ID | 10A6C2 |
| Ig Type | Mouse IgG1 |
| Formulation | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Cynomolgus CD32a / Fc gamma RIIA (Catalog#90015-C08H; AAL92096.1; Met 1-Ile 211). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Specificity | Cynomolgus CD32a / Fc gamma RIIA |
| Has cross-reactivity in ELISA with Human CD32a No cross-reactivity in ELISA withCynomolgus CD32b Human CD32b Human CD16a Human CD16b Human CD64 Human cell lysate (293 cell line) | |
| Western blot | This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Cynomolgus CD32A in WB. Using a DAB detection system, the detection limit for Cynomolgus CD32A is approximately 20 ng/lane under non-reducing conditions and 8 ng/lane under reducing conditions. |
| Direct ELISA | This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Cynomolgus CD32A. The detection limit for Cynomolgus CD32A is approximately 0.16 ng/well. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>CD32/FCGR2
Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Dendritic Cell Pathogen Recognition/Uptake>>CD32/FCGR2
Immunology>>Cluster of Differentiation>>Granulocyte CD Antigen>>Granulocyte Markers>>CD32/FCGR2
Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Macrophage Markers>>CD32/FCGR2
Immunology>>Fc Receptor>>CD32/FCGR2
Receptors that recognize the Fc portion of IgG (Fcγ Rs) functions in the regulation of immune response and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). Fcγ RI binds IgG with high affinity and functions during early immune responses, whereas Fcγ RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94 - 99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fcγ RII A is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM (immunoreceptor tyrosine-based activation motif)- bearing adapter subunit, Fc Rγ, CD32a (Fcγ RII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
Sino Biological Inc. liefert CD32a / Fc gamma RIIA Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.
Weitere Informationen über CD32a / Fc gamma RIIA Antikörper lesen Sie bitte: http://www.sinobiological.com/CD32a-Fc-gamma-RIIA-Antibody-g-9097.html
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