CD31 / PECAM1 Antikörper

17.02.2012

Produkt-Information von CD31 / PECAM1 Antibody Product Information

Immunogen	Recombinant Human CD31 protein (Catalog#10148-H08H)
Antibody Type	Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
Clone ID	1B2B11G4
Ig Type
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD31 / PECAM1 extracellular domain (rhCD31; Catalog#10148-H08H; Met 1-Lys 601; NP_000433.3). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD31 / PECAM1 Antikörper Usage Guide

Immunochemistry

IHC-P: 5-20 μg/mL

CD31-mamary-gland immunohistochemistry CD31-liver immunohistochemistry

Immunochemical Staining of human CD31 in human mammary gland with Mouse Monoclonal Antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to vascular endothelial cells.

Immunochemical Staining of human CD31 in human liver with Mouse Monoclonal Antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to vascular endothelial cells.

CD31-heart immunohistochemistry

Immunochemical Staining of human CD31 in human heart with Mouse Monoclonal Antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to vascular endothelial cells.
The left panel: tissue incubated with primary antibody;
The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃.Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Verwandte Produkte & Themen von CD31 / PECAM1 Antikörper

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Hintergrund von CD31 / PECAM1 Antikörper

The CD31 adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets, and is the only known member of the CAM family on platelets. PECAM-1 has six Ig-homology domains that share sequence similarity with cellular adhesion molecules within the extracellular region, and an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region. Accordingly, CD31acts as a signaling molecule when appropriately engaged and creates docking sites for the Src homology 2 domain-bearing protein tyrosine phosphatase (SHP)-1 and SHP-2. PECAM-1 mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development. In addition, it has been shown that CD31 expression is up-regulated by LPS stimulation, and might function as a feedback negative regulator of LPS inflammatory response in macrophages.

References

Newman, P.J. et al., 1990, Science. 247:1219-1222.
Sun, J. et al., 1996, J. Biol. Chem. 271: 18561-18570.
Newton-Nash, D.K. and Neman, P.J. et al., 1999, J. Immunol. 163: 682-688.
Rui, Y.X. et al., 2007, J. Immunol. 179: 7344-7351.
Woodfin, A. et al., 2007, Arterioscler. Thromb. Vasc. Biol. 27: 2514-2523.

Sino Biological Inc. liefert CD31 / PECAM1 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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