Cathepsin L / CTSL Antikörper (Antigen Affinität reinigt)

09.03.2012

Produkt-Information von Cathepsin L / CTSL Antikörper

Immunogen	Recombinant Mouse Cathepsin L / CTSL protein (Catalog#50015-M08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Mouse Cathepsin L / CTSL (rM Cathepsin L / CTSL; Catalog#50015-M08H; P06797; Met 1-Asn 334). Cathepsin L / CTSL specific IgG was purified by Mouse Cathepsin L / CTSL affinity chromatography.

Cathepsin L / CTSL Antikörper Usage Guide

Specificity	Mouse Cathepsin L / CTSL
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CTSL in WB. Using a DAB detection system, the detection limit for Mouse CTSL is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CTSL. The detection limit for Mouse CTSL is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von Cathepsin L / CTSL Antikörper

Cathepsin L is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism, and is potent in degrading collagen, laminin, elastin, as well as alpha-1 protease inhibitor and other structural proteins of basement membranes. Like many proteases, Cathepsin L is synthesized as an inactive preproenzyme, and cleavage of the 96-residue proregion is necessary to generate the fully active 221-residue mature enzyme. Studies have demonstrated that cleavage of the proregion occur autocatalytically under acidic conditions. Cathepsin L is synthesized in large amounts and secreted by many malignantly transformed cells, and induced by growth factors and tumor promoters. In addition to its role in protein degradation, evidence has accumulated for the participation of Cathepsin L in various physiological and pathological processes, such as tumor invasion and metastasis, bone resorption, spermatogenesis, and arthritis. Accordingly, Cathepsin L may prove useful as a diagnostic or prognostic marker of human tumor malignancy.

References

Mason, R.W. et al., 1989, Biochem. J. 257: 125-129.
Chauhan, SS. et al., 1991, Cancer. Res. 51: 1478-1481.
Chauhan, SS. et al., 1993. J. Biol. Chem. 268: 1039-1045.
Menard, R et al., 1998, J. Biol. Chem. 273: 4478-4484.
Cailhier, JF. et al., 2008. J. Biol. Chem. 283: 27220-27229.

Sino Biological Inc. liefert Cathepsin L / CTSL Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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