CASP7 / Caspase 7 / MCH3 Protein (His Tag)

04.06.2012

Produkt-Information von CASP7 / caspase 7 / MCH3 Protein

Synonym	RP11-211N11.6, CMH-1, ICE-LAP3, MCH3，CASP7
Protein Construction	A DNA sequence encoding the human CASP7 (P55210-1) (Met 1-Gln 303) was fused with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	E.coli

QC Testing von CASP7 / caspase 7 / MCH3 Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: CASP7 / caspase 7 / MCH3 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Ala 24& Ala 207
Molecular Mass	The full length of recombinant human CASP7 comprises 313 amino acids and has a calculated molecular mass of 35KDa. As a result of proteolytic cleavage, the apparent molecular mass of the protein is approximately 20 & 11 kDa ,corresponding to the N-reminal P20 subunit and the C-teminal p11 subunit respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 20mM HEPES,100mM NaCl, 1mM EDTA, 0.10%Sucrose, 0.1%chaps; pH 7.5. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von CASP7 / caspase 7 / MCH3 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von CASP7 / caspase 7 / MCH3 Protein

Caspase 7, also known as caspase-7 and MCH3, is a member of the cysteine-aspartic acid protease (caspase) family. It is highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. Caspase 7 cannot be detected in the brain. Caspases are involved in the signal transduction pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into two major classes - initiators and effectors. The initiator isoforms (caspases-1,-4,-5,-8,-9,-10,-11,-12) are activated by, and interact with, upstream adaptor molecules through protein-protein interaction domains known as CARD and DED. Effector caspases (-3,-6,-7) are responsible for cleaving downstream substrates and are sometimes referred to as the executioner caspases. Caspase 7 functions in the activation cascade of caspases responsible for apoptosis execution. It cleaves and activates sterol regulatory element binding proteins (SREBPs). It proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Overexpression promotes programmed cell death.

References

Riedl S J. et al., 2001, Cell. 104 (5): 791-800.
Roy N. et al., 1997, EMBO J. 16 (23): 6914-25.
Deveraux Q L. et al., 1997, Nature. 388 (6639): 300-4.

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