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| Immunogen | Recombinant Mouse Carboxypeptidase B1 / CPB1 protein (Catalog#50386-M08H) |
| Antibody Type | Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform ) |
| Clone ID | 117 |
| Ig Type | Rabbit IgG |
| Formulation | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carboxypeptidase B1 / CPB1 (rM Carboxypeptidase B1 / CPB1; Catalog#50386-M08H; NP_083982.1; Met 1-Tyr 415). |
| Specificity | Mouse Carboxypeptidase B1 / CPB1 |
| No cross-reactivity in ELISA with Human CPB1 | |
| Western blot | This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse CPB1 in WB. Using a DAB detection system, the detection limit for Mouse CPB1 is approximately 0.25 ng/lane under non-reducing conditions and 0.125 ng/lane under reducing conditions. |
| Direct ELISA | This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CPB1. The detection limit for Mouse CPB1 is approximately 0.00975 ng/well. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Verwandte Bereiche:
Proteine:
Antikörper:
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Sino Biological Inc. liefert Carboxypeptidase B1 / CPB1 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.
Weitere Informationen über Carboxypeptidase B1 / CPB1 Antikörper lesen Sie bitte: http://www.sinobiological.com/Carboxypeptidase-B1-CPB1-Antibody-g-8317.html
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