Carbonic Anhydrase XIII / CA13 Antikörper (Antigen Affinität reinigt)

27.02.2012

Produkt-Information von Carbonic Anhydrase XIII / CA13 Antikörper

Immunogen	Recombinant Human Carbonic Anhydrase XIII / CA13 protein (Catalog#10461-H08E)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase XIII / CA13 (rh Carbonic Anhydrase XIII / CA13; Catalog#10461-H08E; NP_940986.1; Met 1-His 262). Carbonic Anhydrase XIII / CA13 specific IgG was purified by Human Carbonic Anhydrase XIII / CA13 affinity chromatography.

Carbonic Anhydrase XIII / CA13 Antikörper Usage Guide

Specificity	Human Carbonic Anhydrase XIII / CA13
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA13 in WB. Using a DAB detection system, the detection limit for Human CA13 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CA13. The detection limit for Human CA13 is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von Carbonic Anhydrase XIII / CA13 Antikörper

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase 13, also known as Carbonic anhydrase XIII, CA-XIII and CA13, is a member of the alpha-carbonic anhydrase family. CA13 is expressed in thymus, small intestine, spleen, prostate, ovary, colon and testis. CA13 represents a novel enzyme which may play important physiological roles in several organs.

References

Cox J.D. et al., 2000, Biochemistry. 39: 13687-94.
Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA. 99: 16899-903.
Loiselle F.B. et al., 2004, Am. J. Physiol. 286: C1423-C33.
Lehtonen,J. et al., 2004, J Biol Chem. 279 (4): 2719-27.
Kummola,L. et al., 2005, BMC Cancer. 5: 41.

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