C10orf54 / GI24 Protein (His Tag)

27.12.2012

Sino Biological Inc. liefert C10orf54 / GI24 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

Weitere Informationen über C10orf54 / GI24 Protein (His Tag) lesen Sie bitte: http://www.sinobiological.com/C10orf54-GI24-Protein-g-10254.html

Produkt-Information von C10orf54 / GI24 Protein

Synonym	C10orf54
Protein Construction	A DNA sequence encoding the human C10orf54 (AAH20568.1) (Met1-Ala194) was expressed with a polyhistidine tag at the C-terminus.
Source	Human
Expression Host	Human Cells

QC Testing von C10orf54 / GI24 Protein

Purity	> 95% as determined by SDS-PAGE	SDS-PAGE: C10orf54 protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Phe 33
Molecular Mass	The recombinant human C10orf54 consists of 173 amino acids and predicts a molecular mass of 19.6 KDa. It migrates as an approximately 38-42 KDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von C10orf54 / GI24 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von C10orf54 / GI24 Protein

C10orf54, also known as GI24, is a member of the immunoglobulin superfamily. As a transmembrane molecule, C10orf54 is expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, C10orf54 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.

References

Ota T. et al., 2004, Nat Genet. 36 (1): 40-5.
Deloukas P. et al., 2004, Nature. 429 (6990): 375-81.
Colland F. et al., 2004, Genome Res. 14 (7): 1324-32.