AMPK (G1/B2/A2) Heterotrimer Protein

14.06.2012

Produkt-Information von AMPK (G1/B2/A2) Heterotrimer Protein

Protein name	AMPK, 5' AMP-activated protein kinase, 5' adenosine monophosphate-activated protein kinase. Heterotrimer of alpha 2 catalytic subunit, beta 2 non-catalytic subunit and gamma 1 non-catalytic subunit.
Protein Construction	PRKAG1 (P54619, Met 1-Pro 331), PRKAB2 (O43741, Met 1-Ile 272), and PRKAA2 (P54646, Met 1-Arg 552) with a N-terminal polyhistidine-tagged GST tag.
Source	Human
Expression Host	Baculovirus-Insect cells

QC Testing von AMPK (G1/B2/A2) Heterotrimer Protein

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: AMPK (G1/B2/A2) Heterotrimer protein
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA2) has a calculated molecular mass of 158 (38+30+90) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from a 0.2μm filtered solution of 50mM Tris ,200mM NaCl,1mM EDTA ,1mM DTT, 0.5mM PMSF, 10%gly, 1mM GSH,pH 7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von AMPK (G1/B2/A2) Heterotrimer Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von AMPK (G1/B2/A2) Heterotrimer Protein

AMP-activated protein kinase (AMPK) is an important metabolic stress-sensing protein kinase induced by AMP. It can be detected in a number of tissues, including the liver, brain, and skeletal muscle. AMPK is a heterotrimer comprised by three proteins (α, β, and γ subunits) that together make a functional enzyme, conserved from yeast to humans. Seven known genes encode the subunit isoforms (α1, α2, β1, β2, γ1, γ2, γ3) and all are expressed in the heart. The most common isoforms expressed in most cells are the α1(PRKAA1), β1(PRKAB1), and γ1(PRKAG1) isoforms. AMPK induces a cascade of events within cells in response to the ever changing energy charge of the cell. Mammalian AMPK is a stable alphabetagamma heterotrimer comprising a catalytic alpha (PRKAA) and two non-catalytic subunits, beta (PRKAB) and gamma (PRKAG). AMPK activation results in the stimulation of hepatic fatty acid oxidation and ketogenesis, inhibition of cholesterol synthesis, lipogenesis, and triglyceride synthesis, inhibition of adipocyte lipolysis and lipogenesis, stimulation of skeletal muscle fatty acid oxidation and muscle glucose uptake, and modulation of insulin secretion by pancreatic beta-cells.

References

Adams J. et al., 2004, Protein Sci. 13 (1): 155-65.
Stapleton D. et al., 1996, J Biol Chem. 271 (2): 611-4.
Hallows KR. et al., 2009, Am J Physiol. 296 (4): C672-81.
Winder WW. et al., 1999, AmJPhysiol. 277 (1Pt1): E1-10.

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