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| Immunogen | Recombinant Human ADAM15 protein (Catalog#10517-H08H) |
| Antibody Type | Mouse Monoclonal Antibody ( Mouse mAb Service Platform ) |
| Clone ID | 1B7G8 |
| Ig Type | Mouse IgG1 |
| Formulation | 0.2 μm filtered solution in PBS with 5% trehalose |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ADAM15 (rh ADAM15; Catalog#10517-H08H; NP_997074.1; Met 1-Thr 696). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Specificity | Human ADAM15 |
| No cross-reactivity in ELISA with Mouse ADAM9 Mouse ADAM15 Human cell lysate (293 cell line) | |
| Western blot | This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human ADAM15 in WB. Using a DAB detection system, the detection limit for Human ADAM15 is approximately 2 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions. |
| Direct ELISA | This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human ADAM15. The detection limit for Human ADAM15 is 0.0195 ng/well. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Related Areas:
Proteins:
Antibodies:
The adamalysin (ADAM) metalloproteinase disintegrins, also known as metalloprotease/disintegrin/cysteine-rich proteins, are a branch of the metzincin metalloproteinase superfamily that are related to snake venom metalloproteinases and integrin ligands. Human ADAM15 was first named metargidin, and uniquely, it carries an RGD binding motif in a position similar to that in snake venom disintegrins. ADAM15 is widely expressed in various tissues and cells, including human umbilical vein endothelial cells and smooth muscle cells, and its expression can be drived by Vascular endothelial (VE)-cadherin. Overexpression of ADAM15 in NIH3T3 cells appears to enhance cell-cell interactions, as suggested by decreased cell migration, altered cell morphology at the wound edge, decreased monolayer permeability, and increased cell adhesion to monolayers of cells expressing ADAM15 by retroviral transduction. ADAM15 plays a physiological roleas a natural binding partner of integrin αvβ3 thereby loosening tumor cell adhesion to the underlying matrix and regulating tumor cell migration and invasion, and functional interplay between ADAM15 and Src family PTKs may contribute to signaling in hematopoietic cells. Aslo, ADAM15 is believed to interact with integrins αvβ3, α5β1, and α9β1 through its disintegrin domain. ADAM15 has been shown to degrade collagens I and IV and to cleave the inflammatory cytokine CD23, thus influence ECM remodeling within rheumatoid synovial tissues and in atherosclerosis.The high expression levels of ADAM15 correlated with advanced metastatic diseases, and a clear association with clinical parameters was determined.
Sino Biological Inc. liefert ADAM15 Antikörper mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.
Weitere Informationen über ADAM15 Antikörper lesen Sie bitte: http://www.sinobiological.com/ADAM15-Antibody-g-8689.html
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