Acetylcholinesterase / ACHE Antikörper (Antigen Affinität reinigt)

29.02.2012

Produkt-Information von Acetylcholinesterase / ACHE Antikörper

Immunogen	Recombinant Mouse Acetylcholinesterase / ACHE protein (Catalog#50543-M08H)
Antibody Type	Rabbit Polyclonal Antibody ( Antibody Purification Platform )
Ig Type	Rabbit IgG
Formulation	0.2 μm filtered solution in PBS with 5% trehalose
Preparation	Produced in rabbits immunized with purified, recombinant Mouse Acetylcholinesterase / ACHE (rM Acetylcholinesterase / ACHE; Catalog#50543-M08H; NP_033729.1; Met 1-Leu 614). Acetylcholinesterase / ACHE specific IgG was purified by Mouse Acetylcholinesterase / ACHE affinity chromatography.

Acetylcholinesterase / ACHE Antikörper Usage Guide

Specificity	Mouse Acetylcholinesterase / ACHE
Western blot	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ACHE in WB. Using a DAB detection system, the detection limit for Mouse ACHE is approximately 1 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
Direct ELISA	This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ACHE. The detection limit for Mouse ACHE is approximately 0.00975 ng/well.
Storage	This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

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Hintergrund von Acetylcholinesterase / ACHE Antikörper

Acetylcholinesterase, also known as ACHE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission. ACHE is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms, which possess similar catalytic properties, but differ in their oligomeric assembly and mode of attachment to the cell surface. ACHE interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers. ACHE has a very high catalytic activity. Each molecule of ACHE degrades about 25000 molecules of acetylcholine per second. The choline produced by the action of ACHE is recycled. It is transported, through reuptake, back into nerve terminals where it is used to synthesize new acetylcholine molecules.

References

Wilson M.D. et al., 2001, Nucleic Acids Res. 29: 1352-1365.
Yang L. et al., 2002, Neurosci. Res. 42: 261-268.
Hillier L.W. et al., 2003, Nature. 424: 157-164.
Ota T. et al., 2004, Nat. Genet. 36: 40-45.

Sino Biological Inc. liefert Acetylcholinesterase / ACHE Antikörper (Antigen Affinität reinigt) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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