ABCD-1 / CCL22 Protein (His Tag)

16.04.2012

Produkt-Information von ABCD-1 / CCL22 Protein

Synonym	A-152E5.1, ABCD-1, DC/B-CK, MDC, SCYA22, STCP-1
Protein Construction	A DNA sequence encoding the secreeted form of human CCL22 (O00626) (Gly 25-Gln 93 ) was expressed, with a polyhistidine tag at the N-terminus.
Source	Human
Expression Host	E.coli

ABCD-1 / CCL22 Protein QC Testing

Purity	> 90 % as determined by SDS-PAGE	SDS-PAGE: ABCD-1 / CCL22 protein
Endotoxin	Please contact us for more information.
Stability	Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N terminal	Met
Molecular Mass	The recombinant human CCL22 consisting of 84 amino acids and has a calculated molecular mass of 9.7 KDa. It migrates as an 10 kDa band in SDS-PAGE under reducing conditions.
Formulation	Lyophilized from 0.2μm filtered solution of 30% Acetonitrile，0.1% TFA Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.

Usage Guide von ABCD-1 / CCL22 Protein

Storage	Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution	A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Beschreibung von ABCD-1 / CCL22 Protein

Chemokine (C-C motif) ligand 22（ABCD-1 / CCL22）is a kind of CC chemokine which is a family of secreted proteins involved in immunoregulatory and inflammatory processes. The cytokine displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. This ABCD-1 / CCL22 chemokine binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated/effector T lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. ABCD-1 / CCL22 is highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus, and in Langerhans’ cell histiocytosis and atopic dermatitis but not in dermatopathic lymphadenopathy. It is also found in lymph node, appendix, activated monocytes, resting and activated macrophages. This protein is lower expressed in lung and spleen and very weekly expressed in small intestine. The involvement of the macrophage-derived chemokine ABCD-1 or CCL22 and its specific receptor CC chemokine receptor 4 (CCR4) in the formation of T cell-DC clusters has been reported. Reverse transcriptase-polymerase chain reaction analysis revealed high levels of mRNA expression for ABCD-1 or CCL22 in inflamed skin and neck lymph nodes, but not in normal skin.

References

Vulcano M. et al. 2001, Eur J Immunol. 31 (3): 812-22.
Kwon DJ. et al. 2011, Biochem Biophys Res Commun. 417 (4): 1254-9.
Hirota T. et al. 2011, PLoS One. 6 (11): e26987.

Sino Biological Inc. liefert ABCD-1 / CCL22 Protein (His Tag) mit höherer Qualität und günstiger Kosten im Vergleich mit anderen globalen Lieferanten.

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